Guanidine thiocyanate Guanidine thiocyanate (GTC) is a stronger protein denaturant than guanidine hydrochloride and is the denaturant of choice for the preparation of RNA from sources enriched in RNase activity, especially pancreatic tissue (Chirgwin et al, 1979)Guanidine thiocyanate is a powerful, chaotropic agent which is widely used for purification of proteins and nucleic acids Guanidine thiocyanate is preferred for the purification of RNA because it dissociates the RNA into its nucleic acids and protein forms and is commonly used in the guanidinium thiocyanatephenolchloroform extraction methodSIGMA Guanidine thiocyanate BioUltra, for molecular biology, >=990% (AT) MilliporeSigma Synonym(s) Guanidinium rhodanide;
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Guanidine isothiocyanate sigma-Synonym Guanidine isothiocyanate, Guanidinium isothiocyanate, Guanidinium rhodanide, Guanidinium thiocyanate Linear Formula NH 2 C(=NH)NH 2 · HSCN Molecular WeightThe guanidinium isothiocyanate method for DNA isolation was originally developed by Pitcher et al (19) Their method includes the following steps lysis using GuSCN (guanidinium isothiocyanate, EDTA, sarkosyl), extraction with ammonium acetate and chloroform2pentanol mixture, DNA precipitation with isopropanol, and, finally,
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Strong sequestering and denaturation reagent guanidine thiocyanate is also known as guanidine isothiocyanate, guandinium cyanate, guandine thiocyanate, guanidinium rhodanide, thiocyanic acid with guanidine and guanidine, monothiocyanate , with an acronym of GITC It is the essential reagent for nucleic acids sample preparation in molecularGUANIDINE THIOCYANATE Page 1 of 6 Effective Date Initial Release NONEMERGENCY TELEPHONE 24HOUR CHEMTREC EMERGENCY TELEPHONE SDS – SAFETY DATA SHEET 1 Identification Product Identifier GUANIDINE THIOCYANATE Synonyms Guanidine Isothiocyanate Chemical Formula NH2C(NH) NH2 HSCNStructure, properties, spectra, suppliers and links for XL,
Molecular Formula C2H6N4S Synonyms Guanidinium thiocyanate Guanidinium rhodanide Thiocyanic acid, compd with guanidine (11) guanidine;thiocyanic acid MFCDGuanidine and germine These two compounds are briefly referred to on pages 29 and 28 Germine is a veratrum alkaloid and has had occasional use in the form of its mono or diacetate The mechanisms of action of guanidine and germine are not completely understoodThis method relies on the strong chaotropic nature of the reagents involved to completely denature any ribonuclease (RNase) present in the sample After lysis in guanidine thiocyanate buffers there are two possibilities for isolation of the RNA One method involves a series of differential precipitation steps in guanidine hydrochloride (1)
Synonym Guanidine isothiocyanate, Guanidinium isothiocyanate, Guanidinium rhodanide, Guanidinium thiocyanate Linear Formula NH 2 C(=NH)NH 2 · HSCN Molecular Weight11/1/01 · The commonly employed DNA isolation methods involve the use of enzymes such as lysozyme and proteinase K, cetyltrimethylammonium bromide (CTAB) treatment and extraction with organic solvents , detergentinduced lysis in conjunction with proteinase K and lysozyme or lysis using guanidinium isothiocyanate (GITC)containing solutions 3, 4H302 H312 H332, H314, H412 ≤100% Section 4 First Aid Measures
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Guanidine isothiocyanatecontaining reagents with bleach By Samuel Paik, Xiaomao Wu INTRODUCTION We conducted this study in response to concerns regarding the safe handlingUltraPure™ Guanidine Isothiocyanate is a strong protein denaturant when used at high concentrations It is useful in the isolation of intact RNA from sources rich in RNase such as pancreas (1) For Research Use Only Not for use in diagnostic procedures・川口，尼崎倉庫の在庫は即日，その他の倉庫は2〜3営業日以内の出荷となります。 川口，尼崎倉庫からの配送対象エリア は各々異なります。 ・納期に関するご質問につきましては営業部までお問い合
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Guanidinium thiocyanate for synthesis CAS , EC Number , chemical formula H₂NC(NH)NH₂ * HSCN Find MSDS or SDS, a COA, data sheets and more informationHazard classification & labelling Hazard classification and labelling The 'Hazard classification and labelling' section shows the hazards of a substance based on the standardised system of statements and pictograms established under the CLP (Classification Labelling and Packaging) Regulation The CLP Regulation makes sure that the hazards presented by chemicals are clearlyGuanidine isothiocyanate C2H5N4S CID structure, chemical names, physical and chemical properties, classification, patents, literature, biological activities, safety/hazards/toxicity information, supplier lists, and more
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SIGMA Guanidine thiocyanate BioReagent, for molecular biology MilliporeSigma Synonym(s) Guanidinium rhodanide;Guanidine hydrochloride causes decrease in bone marrow activity usually shown as gastrointestinal disturbance and "pins and needles" in the lips, face and extremities Irritability, tremor, incoordination and seizures can occur, rarely, there is low blood pressure, skin reactions, low blood glucose and increased levels of creatinineGuanidium is a chaotrope ie it increases the entropy in the solution It doesn't disrupt hydrophobic interactions Guanidium and urea act by forming hydrogen bonds They can bond with both the dissolved macromolecule and water
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The ratio of TRI(guanidine thiocyanate)reagent and chloroform should be proper during RNA isolationif TRI will be high ,this type of problem may comeyou canIn a highly concentrated guanidine isothiocyanate solution, many proteins unfold and separate into their constituent polypeptides Guanidine Isothiocyanate is widely used to purify nucleic acids from cell extracts Due to its properties as an RNase inhibitor, Guanidine Isothiocyanate Solution is especially useful for the isolation of RNAs from sources rich in RNase, such as pancreasDescription Guanidine thiocyanate is a compound that is widely used for the isolation and extraction of RNA in biochemical research In this capacity, it has been used in many studies relating to cell function, signaling and gene expression Guanidine thiocyanate can also be used to create whole blood lysates for longterm storage or transportation of samples
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Aminomethanamidine thiocyanate, Guanidinium thiocyanate Stronger chaotropic agent than Guanidine Hydrochloride Used in total RNA preparation as 'guanidinium solution' Guanidine Thiocyanate is used as a common protein denaturant in the purification and extraction of mRNA, nucleic acids and proteins from cellularLe thiocyanate de guanidinium ou GITC est un sel composé des cations guanidinium, CH 6 N 3 qui est l'acide conjugué de la guanidine et d'anions thiocyanateIl est principalement utilisé en biochimie comme agent de dénaturation des protéines général, donc comme agent chaotropique bien qu'il soit le plus communément utilisé pour l'extraction d'ADN et d'ARN 4USA US0875A USA USA US A US A US A US 0875 A US0875 A US 0875A US A US A US A US A US A US A Authority US United States Prior art keywords guanidine thiocyanate melamine ammonia guanidine thiocyanate Prior art date 1950
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The lowest effect level was the EC50 of 424 mg/L from an acute study in daphnia Guanidine Thiocyanate is inherently biodegradable and has a log Kow of 11 The criterion of "rapid degradability" is not met as the biodegradation observed inAvantor ®, a Fortune 500 company, is a leading global provider of missioncritical products and services to customers in the biopharma, healthcare, education & government, and advanced technologies & applied materials industriesOur portfolio is used in virtually every stage of the most important research, development and production activities in the industries we serveGuanidine thiocyanate is a water and ethanol soluble reagent used to study protein denaturation, cell signaling and function, and gene expression Guanidine thiocyanate chemical structure It is commonly used in high concentrations to extract and isolate RNA samples because it has the ability to inhibit RNase
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The Quality Is In The Eye Of The Beholder The Perspective Of Ftir And Uv Resonant Raman Spectroscopies On Extracted Nucleic Acids Zucchiatti 18 Journal Of Raman Spectroscopy Wiley Online Library
Here each and every guanidine isothiocyanate are made with reasonable care and all these are nature friendly Also, enjoy a huge collection of exotic and rare versions of these products You can find up to 50% off on authentic guanidine isothiocyanate from the top and best selling brands in the world exclusively at this platformGuanidine Isothiocyanate, supplied by Millipore, used in various techniques Bioz Stars score 97/100, based on 19 PubMed citations ZERO BIAS scores, articleSynonyms Guanidine Isothiocyanate Formula NH 2 C(NH)NH 2 ·HSCN Molecular Weight g/mol CAS# EC# Component Classification Concentration Guanidine Thiocyanate Acute Tox 4;
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Thiocyanic acid, compound with Guanidine (11) Recommended Use Laboratory chemicalsGuanidine Thiocyanate CAS Calbiochem SDS Safety Data Sheet for Guanidine Thiocyanate CAS Calbiochem 3675 Material Safety Data Sheet or SDS for Guanidine Thiocyanate CAS Calbiochem 3675 from MilliporeSigma for download or viewing in the browserNC CASNo Synonyms Guanidinium isothiocyanate;
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Avantor ®, a Fortune 500 company, is a leading global provider of missioncritical products and services to customers in the biopharma, healthcare, education & government, and advanced technologies & applied materials industriesOur portfolio is used in virtually every stage of the most important research, development and production activities in the industries we serve4/22/ · Guanidine thiocyanate Synonyms EINECS RN Acide thiocyanique – guanidine (11) French ACD/IUPAC Name Guanidine isothiocyanate Guanidine thiocyanate Guanidine thiocyanate (11) ACD/IUPAC Name Guanidine thiocyanate solution Guanidine, thiocyanate (11) ACD/Index Name Guanidinium isothiocyanate GuanidiniumGuanidinium thiocyanate Empirical Formula CH 5 N 3 CHNS Linear Formula NH 2 C(=NH)NH 2 HSCN Formula Weight MDL Number MFCD Purity ≥990% (AT) Melting Point 1171 °C;
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Product Name Guanidine thiocyanate Cat No BP2211;Watson International Ltd develops Guanidine thiocyanate CAS 98%min as a chemical compound used as a general protein denaturant Dun & Bradstreet DUN ® Number Use "watltd","watbiz" or "watbz" for quick visitGuanidinium thiocyanate Empirical Formula CH 5 N 3 CHNS Linear Formula NH 2 C(=NH)NH 2 HSCN Formula Weight MDL Number MFCD Melting Point 1151 °C;
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Product Description Guanidine Thiocyanate (Guanidinium thiocyanate or guanidinium isothiocyanate, GITC), a powerful protein denaturant, is most often used to inactivate endogenous RNases in the isolation of RNA from various tissues and bacteria When used with acidequilibrated phenol or phenol chloroform, a solution of Guanidine Thiocyanate and betamercaptoethanolGuanidine monothiocyanate, Guanidine isothiocyanate, Guanidinium isothiocyanate, Guanidinium rhodanide, Guanidinium thiocyanate CAS Catalog Number 1290 100 g 1291 500 g 1292 1 kg Storage Store at 28°C Directions forGuanidinium Isothiocyanate CAS Molecular Formula C 2 H 6 N 4 S Synonym Guanidinium Isothiocyanate Description For the isolation of intact RNA from cell extracts A more powerful denaturing agent than Guanidine Hydrochloride, Guanidine Thiocyanate is suitable for the isolation of intact RNA from cell extracts
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Guanidine isothiocyanate Guanidine rhodanide Guanidinium thiocyanate The following companies are not suppliers of Guanidine thiocyanate This companies are suppliers for equal products with the same CAS number CAS Supplier for Guanidine isothiocyanate (CAS )Guanidine isothiocyanate, and other proprietary components which facilitate the isolation of a variety of RNA species of large or small molecular size TRIzol™ Reagent maintains the integrity of the RNA due to highly effective inhibition of RNase activity while disrupting cells and dissolving cell components during sample homogenizationIdine isothiocyanate procedure of Chirgwin et al (1) as follows The cell pellet was resuspended by vortexing in 5 volumes of a solution of 5 Mguanidine isothiocyanate (BethesdaResearchLaboratories)50 mMTris chloride (pH 76)10 mMEDT%Slauryl sarcosinate (Sigma Chemical Co)140 mM2mercaptoethanol A1g amountofCsCl wasadded to each 25
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To prevent DNA or RNA denaturation, the chaotropic agent, Guanadine thiocyanate is the preferred agent for use during cell lysis, and for DNA or RNA extraction This is because of its strongGuanidine Thiocyanate is an ultrapure, molecular biology grade reagent It is free of detectable nuclease and protease activity and is a strong protein denaturant, as both the guanidinium cation and the thiocyanate anion are chaotropic agents It is provided in
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